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  • Edden
    Junior Member
    • May 2012
    • 7

    #1

    cuffcompare -CG CDS entry lost

    Hi,

    a problem with GFF files.

    I using an "unusal" Gff file:



    In order to use cufflinks with this annotation, I used

    Code:
    cuffcompare -s genome_seqs.fa -CG -r annotation.gtf annotation.gtf
    This produced a GFF which contains both, tss_id's and p_id's, but no cds entries. Strangely, this new GFF only has exon entries and no CDS entries (even though the original has both)
    In the next steps (cufflinks, cuffmerge, cuffcompare,) I don't have any p_id's which finally results in empty cds test when using cuffdiff.

    Any idea why I loose CDs entries?
  • Edden
    Junior Member
    • May 2012
    • 7

    #2
    Any suggestions?

    Comment

    • jb2
      Member
      • Jun 2010
      • 25

      #3
      I'm also interested in a potential answer to this. I've merged a few gtf annotation files together using cuffcompare. These all have p_ids, and also lose the CDS data from the original gtf files. However, when using these union gtf files with cuffmerge, the p_ids are then lost because there is no CDS entries in the union gtf file. Anyone know if there is anyway to keep the p_id entries when doing the above?

      Comment

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