Originally posted by Victory
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Please be more verbose. What commands are you executing to install samtools after copying the SAM folder. You may have to get your cluster administrator to install samtools cluster-wide. I typically have a local install directory that is included in my PATH environmental variable.
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You have to copy the binary to an directory where *other* binaries are installed (like /usr/local/bin). You have to make sure that your PATH environment variable includes the above path. Then you can just "samtools" instead of the "/home/<ETC>/samtools"Originally posted by Victory View PostI use the import command to make BAM file.
samtools import <in.ref_list> <in.sam> <out.bam>
Do I have to use specific command to install the SAM program or just put the program folder on the directory?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...-
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07-31-2026, 11:01 AM -
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