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  • AllPaths-LG error

    I run ALLPATH-LG for staph.tar.gz, .prepare.sh went fine but ./assemble.sh retuned

    Unable to find optional long jumping reads (>20kb) for scaffolding.
    Unable to find optional long reads for patching. [These are optional, I don't think this is the main issue]

    I'm a newbie. Did I pick the wrong data?
    Thanks!!
    Last edited by liviu; 05-31-2012, 07:23 AM.

  • #2
    Is there a configuration file. And if yes, does it refer to this 20 kb library ?

    Comment


    • #3
      Is this the data from GAGE (http://gage.cbcb.umd.edu/data/index.html)? There is no 20 kb library for that, and as you can see, it's optional anyway.

      What kind of scripts are you using? Where are they from? If assemble.sh didn't work, the reason is something else than that error message you posted.

      Comment


      • #4
        Originally posted by liviu View Post
        I run ALLPATH-LG for staph.tar.gz, .prepare.sh went fine but ./assemble.sh retuned

        Unable to find optional long jumping reads (>20kb) for scaffolding.
        Unable to find optional long reads for patching. [These are optional, I don't think this is the main issue]

        I'm a newbie. Did I pick the wrong data?
        Thanks!!
        I Have the same problem when I run ALLPATH-LG. I'm trying to assemble PacBio sequences and when I run the script PrepareAllPathsInputs.pl it reports these warnings and errors:



        ==================== WARNINGS ====================

        !!!! No 'frag' cached read groups found.
        YOU CAN'T RUN AND ASSEMBLY WITHOUT FRAGMENT READS!
        Remember, fragment libraries must have empty 'insert_size' and 'insert_stddev' in the 'in_libs.csv'.


        !!!! No 'jump' cached read groups found.
        YOU CAN'T RUN AND ASSEMBLY WITHOUT JUMPING READS!
        Remember, jumping libraries must have 'insert_size' and 'insert_stddev' defined in the 'in_libs.csv'.


        !!!! No 'long_jump' cached read groups found.
        Long jumping reads (typically 40 kb, < 1x coverage) are useful only for scaffolding of vertebrate size genomes, and are not required for an assembly.

        ==================================================

        ---- 2013-04-29 11:02:08 (CTAPI): Creating '/scratch/hpc/raquel/allpaths/data/ploidy' with PLOIDY = 1.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.fastb'. You can't run an assembly without this file.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.qualb'. You can't run an assembly without this file.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.pairs'. You can't run an assembly without this file.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.fastb'. You can't run an assembly without this file.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.qualb'. You can't run an assembly without this file.
        **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.pairs'. You can't run an assembly without this file.
        **** 2013-04-29 11:02:08 (CTAPI): Found 6 errors.
        ---- 2013-04-29 11:02:08 (CTAPI): Done.

        ---- 2013-04-29 11:02:08 (PAPI): Done.

        Comment


        • #5
          Originally posted by rdlady View Post
          I Have the same problem when I run ALLPATH-LG. I'm trying to assemble PacBio sequences and when I run the script PrepareAllPathsInputs.pl it reports these warnings and errors:



          ==================== WARNINGS ====================

          !!!! No 'frag' cached read groups found.
          YOU CAN'T RUN AND ASSEMBLY WITHOUT FRAGMENT READS!
          Remember, fragment libraries must have empty 'insert_size' and 'insert_stddev' in the 'in_libs.csv'.


          !!!! No 'jump' cached read groups found.
          YOU CAN'T RUN AND ASSEMBLY WITHOUT JUMPING READS!
          Remember, jumping libraries must have 'insert_size' and 'insert_stddev' defined in the 'in_libs.csv'.


          !!!! No 'long_jump' cached read groups found.
          Long jumping reads (typically 40 kb, < 1x coverage) are useful only for scaffolding of vertebrate size genomes, and are not required for an assembly.

          ==================================================

          ---- 2013-04-29 11:02:08 (CTAPI): Creating '/scratch/hpc/raquel/allpaths/data/ploidy' with PLOIDY = 1.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.fastb'. You can't run an assembly without this file.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.qualb'. You can't run an assembly without this file.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/frag_reads_orig.pairs'. You can't run an assembly without this file.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.fastb'. You can't run an assembly without this file.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.qualb'. You can't run an assembly without this file.
          **** Can't find '/scratch/hpc/raquel/allpaths/data/jump_reads_orig.pairs'. You can't run an assembly without this file.
          **** 2013-04-29 11:02:08 (CTAPI): Found 6 errors.
          ---- 2013-04-29 11:02:08 (CTAPI): Done.

          ---- 2013-04-29 11:02:08 (PAPI): Done.

          I know this might be a late response, but I believe that there is a problem with your in_libs.csv file. Have you got it to work since?

          Comment


          • #6
            Actually, I've sent a message to Dr. Lander and Dr. Jaffe, the authors of AllPaths-LG. I asked wether my data is suitable for this tool (PacBio and IonTorrent sequences from an enrichment culture) and how should I run this tool.

            David Jaffe replied me. He said that unfortunately my data is not supported by their program and I should try something else with another tool. Plus he suggested me to join the google groups forum of AllPaths, so I could find some information.
            I've sent a request to join the group 3 weeks ago but they didn't replied me until now.

            So I lost hope in Allpaths-LG and now I'm trying to use IDBA_UD and MIRA assemblers for my data. They are still running the assembly.

            So my conclusion is that AllPathsLG doesn't support hybrid assembly of PacBio+IonTorrent.

            Please, let me know if you know any other programs that could work for this data type.

            Comment


            • #7
              my allpaths exiting with error message in the que and the log file is empty no error msgs also somebody help me to solve this issue thanks in advance


              my pbs script
              #!/bin/bash
              #PBS -l walltime=48:00:00
              #PBS -N 268_allpaths
              #PBS -q workq
              #PBS -l select=40:ncpus=16:mpiprocs=16
              #PBS -l place=scatter:excl
              #PBS -V

              # comment begins with # followed by space......[IMPORTANT]
              # Go to the directory from which you submitted the job
              # cd $PBS_O_WORKDIR

              module load all_paths-2.2
              # path of all_paths
              # path : /app/allpathslg
              module load openmpi-1.6.4


              # ulimit (stack) is needed by the allpaths program
              ulimit -s 100000

              # prepare data for allpaths:
              PrepareAllPathsInput\
              DATA_DIR=$PWD/scratch/268_allpaths\
              PLOIDY=1\
              IN_GROUPS_CSV=/scratch/268_allpaths/in_groups.csv\
              IN_LIBS_CSV=/scratch/268_allpaths/in_libs.csv\
              OVERWRITE=True\
              | tee prepare.out

              # Assemble data:
              allpathslg\
              PRE=$PWD\
              DATA_SUBDIR=data\
              RUN=run\
              SUBDIR=test\
              OVERWRITE=True\
              | tee -a assemble.out


              my csv files

              in_groups.csv

              file_name, library_name, group_name
              /scratch/268_allpaths/SO_2511_268_R1.fastq, illumina, frags
              /scratch/268_allpaths/SO_2511_268_R2.fastq, illumina, frags
              /scratch/268_allpaths/SO_2511_268_R1.fastq.gz, illumina_short, jumping
              /scratch/268_allpaths/SO_2511_268_R2.fastq.gz, illumina_short, jumping

              in_libs.csv

              library_name,project_name,organism_name,paired,frag_size,insert_size,read_orientation,genomic_start,genomic_end
              illumina,testassembly,268,1,300bp,480bp,inward,0,0
              illumina,testassembly,268,1,300bp,480bp,inward,0,0
              illumina,testassembly,268,1,300bp,2k,outward,0,0
              illumina,textassembly,268,1,300bp,2k,outward,0,0


              this is my command line still my assembly is not running, thanks alot for help
              Last edited by madhubioinfo; 05-31-2014, 04:53 AM.

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