I ran TopHat and the run was successful but I am unable to figure out why my junction.bed file is empty? can anybody guide?
thanx
thanx
-
Last edited by bharati; 06-05-2012, 03:54 AM. -
You'll need to provide a lot more information if you want an answer to that question. -
more information ?? like what?Comment
-
can u pls elaborateComment
-
Organism and platform used for sequencing. Did the Tophat run produce any errors? Do you have split reads in your alignment?Comment
-
actually i used the given command:
/home/tophat-2.0.0.Linux_x86_64/tophat -r -34 --mate-std-dev 36 /home/bowtie2-2.0.0-beta5/example/hg19/hg19 /data1/bowtie_aln/R1_RB002_zip_cat_trim_4_90_Q30_80_matched.fastq /data1/bowtie_aln/R2_RB002_zip_cat_trim_4_90_Q30_80_matched.fastqComment
-
do I need to specify some more options along with the above command? Did I missed some thing?Comment
-
The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.Today, 06:37 PM -
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
| Topics | Statistics | Last Post | ||
|---|---|---|---|---|
|
0 responses
27 views
0 reactions
|
by SEQadmin2
11-12-2026, 07:59 AM
|
|||
|
0 responses
35 views
0 reactions
|
by SEQadmin2
11-05-2026, 09:25 AM
|
|||
|
2 responses
56 views
0 reactions
|
by kinpict.com
Today, 06:49 PM
|
|||
|
0 responses
175 views
0 reactions
|
by SEQadmin2
10-28-2026, 09:57 AM
|
Comment