Hello community,
I'm new to the genome sequencing game, though I do have a fairly good understanding of genetics and the technology. I'm currently researching glyphosate resistant Giant Ragweed, for which there is NO reference genome, or a reference genome of any reasonably closely related species.
I'm currently enrolled in my 3rd semester of my MSc. I'd like to use RNA-seq as a means of discovering genes that might be involved in the resistance response. Ideally i'd like to do a pairwise comparison of a mature resistant leaf against a susceptible one, as well as a comparison between a unsprayed mature resistant leaf and a sprayed one. The contact I have for the sequencing would be using the Illumina HiSeq platform.
So I have a few questions:
1. Given that a MSc is supposed to be 6 semesters, is this possible or should I consider switching into a PhD?
2. Is this possible at all?
3. Can you recommend software capable of this kind of analysis and how much computing power and RAM will I need to do this (in a reasonable amount of time)?
4. Does anyone know of a giant ragweend genome or closely related genome that I may be unaware of?
Thanks everyone for your help,
Taylor Jeffery
I'm new to the genome sequencing game, though I do have a fairly good understanding of genetics and the technology. I'm currently researching glyphosate resistant Giant Ragweed, for which there is NO reference genome, or a reference genome of any reasonably closely related species.
I'm currently enrolled in my 3rd semester of my MSc. I'd like to use RNA-seq as a means of discovering genes that might be involved in the resistance response. Ideally i'd like to do a pairwise comparison of a mature resistant leaf against a susceptible one, as well as a comparison between a unsprayed mature resistant leaf and a sprayed one. The contact I have for the sequencing would be using the Illumina HiSeq platform.
So I have a few questions:
1. Given that a MSc is supposed to be 6 semesters, is this possible or should I consider switching into a PhD?
2. Is this possible at all?
3. Can you recommend software capable of this kind of analysis and how much computing power and RAM will I need to do this (in a reasonable amount of time)?
4. Does anyone know of a giant ragweend genome or closely related genome that I may be unaware of?
Thanks everyone for your help,
Taylor Jeffery
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