Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • EBER
    Member
    • May 2012
    • 10

    samtools sort

    Hello,
    I am trying to sort a large bam file, actually split in two, with samtools sort
    I use -m 10000000000 so the job creates 8 sorted files of about the same size, plus a smaller last one.
    At the end of the job, samtools sort give the message "core_sort_merge 9 bam files" and it stays like that for a while before the screen starts showing multiple caracters one after the other.

    My question is, can I assume the job was completed?
    My command-line was
    samtools sort -no -m 10000000000 inpunt.bam output.prefix &

    Any help appreciated.
  • adaptivegenome
    Super Moderator
    • Nov 2009
    • 436

    #2
    Probably the best thing to do is just check to make sure you have the same number of reads in the output files as you do in your input.

    Not sure if you are interested but we also have a parallelized implementation of "samtools sort" that combines the merge/sort step. Its at http://www.github.com/adaptivegenome/openge. It's open source and there are also binaries for convenience...

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    100 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    121 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    113 views
    0 reactions
    Last Post SEQadmin2  
    Working...