Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • sigma
    Junior Member
    • May 2012
    • 8

    #1

    Bowtie2 single seq mapping

    Hi friends,

    I'm trying to map some sequences (length : 17) in the initial chromosome sequence.
    So i use this command :

    /usr/local/bowtie2-2.0.0-beta5/bowtie2 -f --end-to-end -x Indexed_CHR10 -U INPUT.fasta -S OUTPUT.sam

    so in INPUT it's a fasta format with my 17-mers
    The problem is that i have warnings and so the mapping is not good.

    Warning: skipping read 'tttttttgcaatagtag' because length (0) <= # seed mismatches (0)
    Warning: skipping read 'tttttttgcaatagtag' because it was < 2 characters long


    advance thanks,
    Sigma
    Last edited by sigma; 06-11-2012, 06:47 AM.
  • sigma
    Junior Member
    • May 2012
    • 8

    #2
    It's working, the problem was really simple :
    actually i've given a bad fasta file format :

    >SEQ

    and not :

    >ID
    SEQ
    ...

    regards,
    Sigma

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...