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  • sigma
    Junior Member
    • May 2012
    • 8

    #1

    Bowtie2 single seq mapping

    Hi friends,

    I'm trying to map some sequences (length : 17) in the initial chromosome sequence.
    So i use this command :

    /usr/local/bowtie2-2.0.0-beta5/bowtie2 -f --end-to-end -x Indexed_CHR10 -U INPUT.fasta -S OUTPUT.sam

    so in INPUT it's a fasta format with my 17-mers
    The problem is that i have warnings and so the mapping is not good.

    Warning: skipping read 'tttttttgcaatagtag' because length (0) <= # seed mismatches (0)
    Warning: skipping read 'tttttttgcaatagtag' because it was < 2 characters long


    advance thanks,
    Sigma
    Last edited by sigma; 06-11-2012, 06:47 AM.
  • sigma
    Junior Member
    • May 2012
    • 8

    #2
    It's working, the problem was really simple :
    actually i've given a bad fasta file format :

    >SEQ

    and not :

    >ID
    SEQ
    ...

    regards,
    Sigma

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