Read up on paired end or mate-pair libraries.
The short answer is, by mapping reads derived from both ends of a molecule, one can "size" that molecule against the reference genome. If you know you made your library with 250bp physical molecules, and the ends of that molecule map 10kb apart in the reference genome, you can infer that your sample genome has a deletion.
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Alignment based sizing of inserts
Hi everyone,
I am new to the field of DNA sequencing. I need a quick help, can anyone explain me what do we mean by alignment based sizing of insert DNA and how different it is from physical size of the DNA. How do we calculate this alignment based size.
A quick help will be really appreciated, I have an impt. meeting tomo where I need this info.
Regards
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The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM
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