Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Alignment based sizing of inserts

    Hi everyone,

    I am new to the field of DNA sequencing. I need a quick help, can anyone explain me what do we mean by alignment based sizing of insert DNA and how different it is from physical size of the DNA. How do we calculate this alignment based size.

    A quick help will be really appreciated, I have an impt. meeting tomo where I need this info.

    Regards

  • #2
    Read up on paired end or mate-pair libraries.

    The short answer is, by mapping reads derived from both ends of a molecule, one can "size" that molecule against the reference genome. If you know you made your library with 250bp physical molecules, and the ends of that molecule map 10kb apart in the reference genome, you can infer that your sample genome has a deletion.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Exploring Human Diversity Through Large-Scale Omics
      by seqadmin


      In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
      Today, 06:43 AM
    • seqadmin
      Best Practices for Single-Cell Sequencing Analysis
      by seqadmin



      While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
      06-06-2024, 07:15 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 06-21-2024, 07:49 AM
    0 responses
    224 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-20-2024, 07:23 AM
    0 responses
    232 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-17-2024, 06:54 AM
    0 responses
    231 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-14-2024, 07:24 AM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Working...
    X