Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • nat
    Junior Member
    • Jul 2009
    • 8

    most commonly used alignment viewers

    Hi there,

    I am trying to get a feel for the mosy commonly used/ well regarded alignment viewers out there. I have had a read of the sticky thread outlining what options are available, but Im interested in what people think are well regarded in terms of:

    ease of use and well supported
    which require local install/ only webtool available
    can you navigate in at the genome level (ie. with Affymetrix IGB viewer)
    which are more suitable for short/long read data - or one that caters for both
    Do they take standard output from commonly used ailgners (velvet/bowtie/maq) - or is there much file manipulation before the viewer can be used.

    Are people using SAMtools much?

    Thanks,

    Natalie
  • zee
    NGS specialist
    • Apr 2008
    • 249

    #2
    I have been using the Integrated Genomics Viewer for my short read work.

    See http://www.broadinstitute.org/igv/index.html

    Heng Li also did a good review of viewers at http://www.sanger.ac.uk/Users/lh3/NGSalnview.shtml


    IGV works nicely with BED, BAM and GFF files. It does the job for most of my tasks.

    Comment

    Latest Articles

    Collapse

    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      Yesterday, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Today, 11:08 AM
    0 responses
    6 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    11 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    52 views
    0 reactions
    Last Post SEQadmin2  
    Working...