Fastq results from MiSeq - which version?

[Lilach]

Hi,
I am trying to analyse fastq files derived from Illumina MiSeq sequencing, and I should known what type of fastq file is it, to calculate the phred scored?
Sanger fastq? Illumina fastq (which version)?

Thanks in advance!

[Heisman]

You can install a program called "FastQC" and run your .fastq files through it. It will tell you what encoding is used.

[Lilach]

thank you very much!

[ulz_peter]

Our MiSeq FastQs are output using Sanger encoding (33 offset to the phred score), which should be the same as Illumina1.8+.

[Lilach]

Thank you, Peter.
Indeed I used the FastQC software that Heisman recommended, and it was Sanger fastq.