DNA sequencers 150 bps Output File

[Arupsss]

I am doing some experiment using BowTie 2. Now, I want, to experiment with it on 150 bps reads. So, I want DNA sequencers 150 bps output file. For example, output of Genome Analyzer IIx. Can anybody please help me, where can I find it ?

[GenoMax]

I think your best bet would be to find a MiSeq data set (rather than a GAIIx). MiSeq will easily do 2 x 150 bp. There seem to be MiSeq runs several available in the SRA (http://sra.dnanexus.com/?result_type...&show=&q=miseq or http://www.ncbi.nlm.nih.gov/sra?term=miseq). Not simple to determine if they are 150 bp so you may need to look through some.

[Arupsss]

Thanks a lot. I go through those links. In their download section they have not specify number of bases (means 100 or 150 etc.). You mean to download and check number of bases ?

[GenoMax]

I *think* you can actually see the length of the reads by clicking on the names of the SRA records.

See the example below. This seems to be a 150 bp paired-end MiSeq run. But them many of the others seem to be as well.

http://www.ncbi.nlm.nih.gov/sra/SRX149549

Thanks a lot. I go through those links. In their download section they have not specify number of bases (means 100 or 150 etc.). You mean to download and check number of bases ?

[GenoMax]

If you need human samples (rather than bacteria) : http://www.ncbi.nlm.nih.gov/sra?term=miseq%20human

[TonyBrooks]

There are also a couple of default 150PE runs if you're registered on Illumina BaseSpace.
Just a PhiX and BacillusCereus run, so I'm not sure how useful that'll be.
FASTQ is downloadable.