Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
It is showing you the base at the 5' position. The '1' means that the forward strand begins at position 1. 5' to 3'. The '151' means that the reverse strand ends at position 151 once again reading 5' to 3'.
BTW: There is no need to repeat your question in multiple threads.
BTW#2. The miSeq puts out 151 base pair reads not 150 bp reads. That is just the way it is.
-
Originally posted by westerman View PostIt is showing you the base at the 5' position. The '1' means that the forward strand begins at position 1. 5' to 3'. The '151' means that the reverse strand ends at position 151 once again reading 5' to 3'.
BTW: There is no need to repeat your question in multiple threads.
BTW#2. The miSeq puts out 151 base pair reads not 150 bp reads. That is just the way it is.
Comment
-
Originally posted by Arupsss View Postmeans first end (1-151) is taken from DNA's forward stand and second one (152-302) taken from DNA's reverse strand ? Means are they reverse complement ? Sorry, I have very little idea about Bioinformatics.
When you eventually download the reads in fastq or fasta format the 'forward' reads will be 5' to 3' bases 1 to 151. The 'reverse' reads should be 5' to 3' bases 151 to 1. Although, when I download the fastq I am getting 150 bases for the forward and 152 bases for the reverse. So using the SRA tools might be a better choice for data manipulation.
Comment
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
26 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
159 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|
Comment