Originally posted by Arupsss
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When you eventually download the reads in fastq or fasta format the 'forward' reads will be 5' to 3' bases 1 to 151. The 'reverse' reads should be 5' to 3' bases 151 to 1. Although, when I download the fastq I am getting 150 bases for the forward and 152 bases for the reverse. So using the SRA tools might be a better choice for data manipulation.
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