Tophat2 fusion post

[MerFer]

Dear Tophat2 user,

After I ran tophat2 with --bowti1 option I am now trying to run tophat-fusion-post.
My directory structure is
- top_dir

-tophat_sample_1
-tophat_sample_2
-ensGene.txt
-refGene.txt
-mcl
-blast (human_genomic* and nt*)

When I run post I get an error like
EXIT CODE 5
FATAL: Argument 1 ("-db") is not recognized or is improperly formed.

Do you have an idea what I am doing wrong?

thanks

[NKAkers]

Why don't you give the command that you tried to run. It sounds like you tried to use a -db option, which isn't supported by tophat-fusion-post.

[MerFer]

the tophat fusion post from Tophat2 uses the newest version of blastn, which has a -db option instead of -d option. That's why I got the errors.

Thank you