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  • ymc
    replied
    Can short read aligner handle fastq with heterozygous nucleotide calls (ie, M, R, S, Y, K, etc)??

    Leave a comment:


  • swbarnes2
    replied
    If you can convert them to fastas, or fastqs, you might be able to use a short-read aligner to ailgn them and make .bams. Or maybe one of tha blast output formats can be converted

    Leave a comment:


  • How to align diploid ab1 files to the reference genome?

    I have some human ab1 chromatogram files. Is it possible to align them to the reference genome and get the bam file? How to do that? Thanks in advance!

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  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
    by seqadmin




    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
    07-08-2024, 03:19 PM

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