Learn how to use Blastx

[angeloulivieri]

Hi, all. I'm new for the forum and for Blast.

I've to use blastx for some sequence matching. I would address my learning in some way but I cannot find nothing specific about its use. I've already read the user guide on NCBI and the man page besides of a lot of googlein.

Someone knows about some simple guide to the commands of blastx?

Thank you all

[yangjianhunt]

usually the ncbi site manual/guide is pretty well written. Could you provide details on the problem you encountered?

[JackieBadger]

use BLAST2GO.... If yo use blastx you can then easily pull up annotations to your sequences

[jimmybee]

There are a good number of BLAST webservers (such as http://blast.ncbi.nlm.nih.gov) which have BLASTX, so why don't you test the program using that. All the parameters (bar a few) are there on the website so you can get a feel for the program. That way when you come to the commandline you know what the parameters are and how to use them. The best way of learning is doing

[angeloulivieri]

There are a good number of BLAST webservers (such as http://blast.ncbi.nlm.nih.gov) which have BLASTX, so why don't you test the program using that. All the parameters (bar a few) are there on the website so you can get a feel for the program. That way when you come to the commandline you know what the parameters are and how to use them. The best way of learning is doing

I'm seeing that there aren't a lot of parameters on the webpage at ncbi... the command line options are a lot more..