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  • How to find eversions with Pysam code?

    I would like to write code to find eversion.

    To find inversion, the code for this if statement is as follows:

    elif read.is_reverse == read.mate_is_reverse:

    But I cannot figure out what the code is for finding eversion.

    Does somebode know this code?

    I tried:

    elif read.is_reverse == True and read.mate_is_reverse == False:

    and

    elif read.is_reverse == False and read.mate_is_reverse == True:

    But both won't work. How can that be?

    Can somebody help me?

  • #2
    Are you checking both the read and its mate are mapped?

    P.S. From a style point of view it is more common to do "if statement:" and "if not statement:" rather than "if statement==True:" and "if statement=False:"

    P.P.S. I think you mean "inversions" not "eversions". Places where the sequence has (somehow) been inverted (turned round).

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