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  • samboy
    Junior Member
    • Jul 2012
    • 1

    #1

    Reconstructing a full genome from 1000 genome project

    Hi there,

    I am trying to reconstruct whole DNA sequence of an individual (HG00096) from 1000 genome project and save it in a fasta file. I have examined ftp.1000genomes.ebi.ac.uk and found unmapped bam files under directory ftp.1000genomes.ebi.ac.uk/vol1/ftp/data/HG00096/alignment for individual HG00096. Although i searched forum, I kinda got stuck at this point.

    1- Is this the right file containing different parts of individual HG00096 from reference?
    2- If so, where can i find the suitable reference genome to work with this bam file?
    3- With which tool can i combine reference genome and unmapped parts of individual HG00096?

    Sorry for the lousy question but i am kinda new in this job.

    Thanks in advance.
  • swNGS
    Member
    • Nov 2011
    • 83

    #2
    All the raw FASTQS that generated the bam file ( the aligned file) should be there too somewhere. It might be helpful if you explained why you are trying to do that.
    I've found it a bit of a minefield, but here are directions on the 1000 genome bite to hopefully point you in the right direction. Failing that, they have a help email address.

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