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Ah. Now that I think about it. I think the SRR035330 run is a pair ended one. But for some reasons, only one end is sequenced for some fragments. This explains why the SRR035330.filt.fastq.gz is much smaller than the other two files.
Did I describe the situation correctly?
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That's the idea. And then of course sort and mark duplicates....
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Thanks for your reply. Suppose I want to generate a single bam file from them. Is this the right way to do this?
bwa aln -t 6 hg19.fa SRR035330.filt.fastq.gz > 0.sai
bwa aln -t 6 hg19.fa SRR035330_1.filt.fastq.gz > 1.sai
bwa aln -t 6 hg19.fa SRR035330_2.filt.fastq.gz > 2.sai
bwa samse hg19.fa 0.sai SRR035330.filt.fastq.gz | samtools view -bS - > se.bam
bwa sampe hg19.fa 1.sai 2.sai SRR035330_1.filt.fastq.gz SRR035330_2.filt.fastq.gz | samtools view -bS - > pe.bam
samtools merge SRR035330.bam se.bam pe.bam
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The third file is unpaired. I'm not sure which genomes you are using but many are sequenced using multiple platforms. For example, some are sequenced using SOLID and ILLUMINA.
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1000g sequence reads PE or SE?
I have trouble understand which reads are single reads and which are pair ended reads
For example, there are three files in
ftp://ftp.1000genomes.ebi.ac.uk/vol1...sequence_read/
SRR035330_1.filt.fastq.gz
SRR035330_2.filt.fastq.gz
SRR035330.filt.fastq.gz
I checked the sequence index and they are all supposedly PAIR_ENDED. I think SRR035330_1.filt.fastq.gz and SRR035330_2.filt.fastq.gz constitute one pair. I think I can run them thru bwa to get two sai files and go from there.
But what is SRR035330.filt.fastq.gz??? Is it really pair ended reads? Or is it single reads?
Thanks a lot!Tags: None
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