Little concordance between GATK and Samtools

[ramirob]

Hello,

I have read that GATK and Samtools should give you about 90% concordance (Hugeseq test). I am not able to reproduce that and was wondering if anyone has done it and can tell me what parameters they used??

I am doing:

java -Xmx4g -jar aln.bam -R ucsc.hg19.fasta --min_base_quality_score 30 -T UnifiedGenotyper -o gatk.vcf

samtools mpileup -uf ucsc.hg19.fasta --Q 30 -D -g -I -S aln.bam | bcftools view -bvcg - > samtools.bcf
bcftools view samtools.bcf > samtools.vcf

Any insight?

Thanks in advance,
Ramiro

[Bukowski]

So what concordance *are* you getting?

[ramirob]

Well, with vcf-compare I am getting about 30%!

# The command line was: vcf-compare(r731) chr10gatk.raw.vcf.gz chr10.samtools.raw.vcf.gz
#
#VN 'Venn-Diagram Numbers'. Use `grep ^VN | cut -f 2-` to extract this part.
#VN The columns are:
#VN 1 .. number of sites unique to this particular combination of files
#VN 2- .. combination of files and space-separated number, a fraction of sites in the file
VN 271 chr10.samtools.raw.vcf.gz (35.4%) chr10gatk.raw.vcf.gz (38.2%)
VN 438 chr10gatk.raw.vcf.gz (61.8%)
VN 494 chr10.samtools.raw.vcf.gz (64.6%)
#SN Summary Numbers. Use `grep ^SN | cut -f 2-` to extract this part.
SN Number of REF matches: 271
SN Number of ALT matches: 270
SN Number of REF mismatches: 0
SN Number of ALT mismatches: 1
SN Number of samples in GT comparison: 0

[Bukowski]

Well, with vcf-compare I am getting about 30%!

# The command line was: vcf-compare(r731) chr10gatk.raw.vcf.gz chr10.samtools.raw.vcf.gz
#
#VN 'Venn-Diagram Numbers'. Use `grep ^VN | cut -f 2-` to extract this part.
#VN The columns are:
#VN 1 .. number of sites unique to this particular combination of files
#VN 2- .. combination of files and space-separated number, a fraction of sites in the file
VN 271 chr10.samtools.raw.vcf.gz (35.4%) chr10gatk.raw.vcf.gz (38.2%)
VN 438 chr10gatk.raw.vcf.gz (61.8%)
VN 494 chr10.samtools.raw.vcf.gz (64.6%)
#SN Summary Numbers. Use `grep ^SN | cut -f 2-` to extract this part.
SN Number of REF matches: 271
SN Number of ALT matches: 270
SN Number of REF mismatches: 0
SN Number of ALT mismatches: 1
SN Number of samples in GT comparison: 0

OK I admit that seems quite low, but do you really want to be considering the raw, unfiltered output for your comparisons?

[ramirob]

Not really, but if I filter the gatk output I would just get less variants wouldn't I? The main question I have is, are the parameters I am using ok? Maybe I can check other alignments. I have been trying to find out what parameters people use that they report 90% concordance, do you know?

Thank you very much for your attention and help!
Ramiro

[Bukowski]

Not really, but if I filter the gatk output I would just get less variants wouldn't I? The main question I have is, are the parameters I am using ok? Maybe I can check other alignments. I have been trying to find out what parameters people use that they report 90% concordance, do you know?

Thank you very much for your attention and help!
Ramiro

Yes you would get less variants, but they would also be high quality ones.

I don't know much about samtools mpileup parameters but assuming you're working on human data, the GATK best practice documents would be a good place to start for refining GATK parameters.

http://www.broadinstitute.org/gsa/wi...th_the_GATK_v3

There's plenty of pipelines on Github and the like with other peoples parameters exposed. I wasn't able to find information on the parameters in the HugeSeq paper, but I note it was for a very old version of GATK.