I would be interested in mapping reads to complete bacterial genomes using bwa-sw or Bowtie2. But I'm unable to index the complete genome file (single FASTA with all bacterial genomes from NCBI) using BWA/Bowtie2 as it reports error on the limit of sequence bases in the reference file.
Can anyone suggest me what is the best approach to accomplish this? I'm not interested in splitting the sequences in 3-4 chunks and created multiple index files, as it would be difficult to extract unmapped reads .
Pl suggest
Raj
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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