When calculate a PRKM for a gene locus
R=109C/NL
where C is the number of mappable reads that fell onto the gene’s exons, N is the total number of mappable reads in the experiment, and L is the sum of the exons in base pairs.
for genes with alternative splicing, like a intron retain, how to determin the L ? is this intron count into the L ?
R=109C/NL
where C is the number of mappable reads that fell onto the gene’s exons, N is the total number of mappable reads in the experiment, and L is the sum of the exons in base pairs.
for genes with alternative splicing, like a intron retain, how to determin the L ? is this intron count into the L ?
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