Does anyone know of depth of coverage or read depth algorithms that incoporates information on heterozygosity (like B allele frequency as is commonly used for SNP array algorithms such as QuantiSNP and PennCNV)?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM