I have three different transcriptome assemblies (Kmer 30,35 & 65) of one insect species. I want to compare transcriptome assemblies with reference insect sequence(from ensembl). I want to know how many genes show up in all kmer assemblies and between individual assemblies (i.e between 30 & 35, between 35 & 65). I would like to get idea to better way to compare to get list of genes. Any journal paper info regarding this will be helpful.
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Hi Bioman1,
Can you share the purpose behind your proposed steps? Please look into whether CD-HIT is the right tool for you.
Regards,
Douglas
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transcriptome assembly-comparative genomics
Thanks for reply. I have transcriptome assemblies with different treatments. I want to know which genes present in all treatments and what are all the genes that are absent in different treatments. I have tried tblastx ensembl (cDNA) insect sequence against transcriptome assemblies. Trying to find out highest coverage. Any suggestion?Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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