next generation sequencing simulation softwares

[me91]

Hi
I need a next generation sequencing simulation software.
I'v worked with "metasim" before but it has lots of bugs and does not work well!!
I would be glad if somebody introduces best softwares.
thank you all

[kamsen]

Have a look at http://www.seqan.de/projects/mason/.

[adaptivegenome]

dgwsim is a good one by nils homer

[rnaseek]

For simulating RNA-Seq data there BEERS by a group from U.PENN.

http://cbil.upenn.edu/BEERS/

[me91]

thank you all

and witch one is suitable for simulating very long reads to get long stream of SNPs ?

[raj.musunuri]

try ART

http://www.niehs.nih.gov/research/resources/software/biostatistics/art/

[Ana Suárez]

Dear all,

I am using MetaSim which doesn't produce .qual files. As explained at the FAQs, all inserted errors/substitutions are reported in the fasta header per read, that look like that:

>r1.1 |SOURCES={GI=320159410,bw,1206304-1206640}|ERRORS={52_1:C,89_1:A,90_1:G,90_2:T,216_1:T}|SOURCE_1="Anaerolinea thermophila UNI-1" (0d34b7871fbf039b0739d6497bb075cffa74a470)

Do you know any way to generate a .qual file from that information?

Many thanks in advance!

[syfo]

You can find more in the wiki