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  • How to merge interval files generated from RealignerTargetCreator

    Hi all - I'm using GATK realigner which can take several hours on my human samples. I'm trying to optimize my pipeline by dividing this up by chromosome for each node in my cluster. I can call RealignerTargetCreator using the -L parameter for each chromosome which results in a bunch of interval files. Now, I either want to call IndelRealigner using the -L parameter for each chromsome then merge the resulting BAM files. My other thought was to merge the interval files into one then call IndelRealigner. So this leads me to 2 questions:

    1) I don't see a way to merge interval files using GATK. Is this possible?

    and

    2) Can I call IndelRealigner and process each chromosome separately then merge the resulting BAM files together?

    Has anyone tried this before?

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