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  • minhduc
    Junior Member
    • Apr 2012
    • 5

    #1

    Help!! Interpret MoDIL results.

    Hi,
    I have been running to MoDIL to locate indels and have problems interpreting the output. According to the README file
    Results format is:

    - Cluster ID: Unique ID for each indel prediction
    - Chromosome: Chromosome where the indel is located.
    - Start location: Start location of indel
    - End location: End location of indel
    - Insertion flag: If it is 1, the row means insertion, deletion otherwise.
    - Deletion flag: If it is 1, the row means deletion, insertion otherwise.
    - Size of indel: If it is negative it means deletion, insertion otherwise.
    (ex) -300 => 300bp deletion
    (ex) 12 => 12bp insertion
    - P value: P-value of the indel prediction
    - Likelihood of homozygosity: If it is homozygous, it is close to 1.
    - Number of matepairs that support indel
    - Number of matepairs that support no indel: If it is zero, the indel
    is homozygous
    and an excerpt from the predicted INDELs in Yoruban genome from MoDIL website:
    1.0 chr1 218651814 218652002 1 0 75.0 0 1e-09 21220038 0 47 0 25 36 218651906 218651972
    1.0 chr1 102759676 102760211 0 1 -329.0 0 1e-09 10323514 0 67 0 32 37 102759712 102760037
    I'd interpret the above results as a 75pb insertion starting at pos 218651814 ending at 218652002, and a 329bp deletion from 102759676 to 102760211. I do not understand how an insertion of size 75bp can start at 218651814 and end at 218652002 (i.e., 188 bp apart)? Likewise, in the second line, how a deletion from 102759676 to 102760211 (535 bp in between) can be a 329pb deletion? Could anyone help to explain or to point out what is wrong with my interpretation?

    Many thanks
    MD

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