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  • krause007
    started a topic Goseq analysis on Arabidopsis

    Goseq analysis on Arabidopsis

    Hello,
    I'm trying to run the goseq on a not supported organism but unfortunately I already stuck for a while with the nullp function...
    I have my DE genes in a vector:
    head(degenes)

    AT1G21660 AT1G11310 AT1G06220 AT1G01010 AT1G01020 AT1G01030
    1 0 0 0 0 0

    and also my gene_length:

    head(gene_length)
    AT1G21660 AT1G11310 AT1G06220 AT1G01010 AT1G01020 AT1G01030
    3366 3447 4909 2268 2809 2065

    if I run now the nullp function:
    pwf<-nullp(degenes,bias.data=gene_length)
    Error in if (hi <= low) { : missing value where TRUE/FALSE needed

    Its not too good explained in the goseq manual so I hope anybody can help me.
    Cheers,
    Julian

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