Hello, I did some tophat mapping and looking at the results, it seems that the strand of the junctions that tophat predicts are very much consistent (I would say 90-95% of the time I see it, the junction strand matches the gene strand) with the actual strands that the gene belongs to. I know that the rna-seq data I have is not stranded. Is there an expectation that the junction strands will match the gene's actual strand? Thanks.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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