Hello, I did some tophat mapping and looking at the results, it seems that the strand of the junctions that tophat predicts are very much consistent (I would say 90-95% of the time I see it, the junction strand matches the gene strand) with the actual strands that the gene belongs to. I know that the rna-seq data I have is not stranded. Is there an expectation that the junction strands will match the gene's actual strand? Thanks.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
