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  • scibetta
    Junior Member
    • Aug 2012
    • 1

    #1

    import reference.fa reference in samtool ubuntu

    Hello everybody,
    I am very new with samtool. I need to convert a sam file generated with bowtie into bam. I need some help from scratch (all the commands in ubuntu to be used) on how to import and index the hg.19.fa reference file from bowtie or internet.
    Please please please help me.
    Angelo
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    I need to convert a sam file generated with bowtie into bam.
    samtools view -bhS yourfile.sam > yourfile.bam

    Poke around the Bowtie site, you can probably find a pre-indexed hg19 that you can just download, since it's a popular genome.

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