Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Tings
    Junior Member
    • Aug 2012
    • 1

    #1

    CONTRA vcf results

    hi, i'm learning the CONTRA, by using the test datas downloaded on sourceforge, command as follows { Python-2.7/python CONTRA.v2.0.3/contra.py -t 0247401_D_BED_20090724_hg19_MERGED.bed -s P0667T_GATKrealigned_duplicates_marked.bam -c P0667N_GATKrealigned_duplicates_marked.bam -f human.fa -o outdir };
    here are the programs in it:
    1. Python-2.7
    2. R version 2.10.0
    3. samtools version: 0.1.8
    4. BEDTools-Version-2.11.2

    No error reports during processing(just like the web shows http://sourceforge.net/projects/cont.../Test%20files/). 3 files in the '/outdir/table' dir (CNATable.10rd.10bases.20bins.vcf; CNATable.10rd.10bases.20bins.DetailsFILTERED.txt; CNATable.10rd.10bases.20bins.txt), there are no cnv information in vcf file, only this description (
    ##fileformat=VCFv4.0
    ##reference=1000GenomesPilot-NCBI36
    ##INFO=<ID=SVTYPE,Number=1,Type=String,Description="Type of structural variant"
    ##INFO=<ID=END,Number=1,Type=Integer,Description="End position of the variant described in this record"
    ##ALT=<ID=CNV,Description="Copy number variable region"
    #CHROM POS ID REF ALT QUAL FILTER INFO)
    dose anyone know why?? thanks for help~
  • jmatés
    Member
    • Jan 2013
    • 14

    #2
    I'm at the same point... does anyone know what's going on?

    Comment

    • dawe
      Senior Member
      • Apr 2009
      • 258

      #3
      I had the same issue and wrote to the developer. He said he would have checked that but there have been no updates since then.
      I've tried to run CONTRA with python debugger, I remember there was a bug in some intermediate file that was not created, but then gave up and worked with bed-like outputs.

      d

      Comment

      • jmatés
        Member
        • Jan 2013
        • 14

        #4
        Originally posted by dawe View Post
        I had the same issue and wrote to the developer. He said he would have checked that but there have been no updates since then.
        I've tried to run CONTRA with python debugger, I remember there was a bug in some intermediate file that was not created, but then gave up and worked with bed-like outputs.

        d
        Thank you dawe.

        It's a pity. The program looks very nice and it fits perfectly to my bunch of samples! I've also written to the developer and well... waiting !

        Anyway if you could shed some light on how to work with CNVs with this "bed-like outputs" I'll be very grateful. One of the hardest things when you're a rookie (I think) is to have good judgment about which tools to use...

        Comment

        • mafonso
          Junior Member
          • Jul 2014
          • 4

          #5
          Hi,

          I have the same issue and can't figure out what the issue is. Did someone solve this problem? Is there another way to get the results without the .vcf file?

          Thanks

          Comment

          Latest Articles

          Collapse

          • kinpict.com
            Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by kinpict.com
            The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
            Today, 06:37 PM
          • SEQadmin2
            16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by SEQadmin2





            NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
            10-30-2026, 06:46 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 11-12-2026, 07:59 AM
          0 responses
          27 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 11-05-2026, 09:25 AM
          0 responses
          35 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 11-03-2026, 11:54 AM
          2 responses
          56 views
          0 reactions
          Last Post kinpict.com  
          Started by SEQadmin2, 10-28-2026, 09:57 AM
          0 responses
          175 views
          0 reactions
          Last Post SEQadmin2  
          Working...