Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Tings
    Junior Member
    • Aug 2012
    • 1

    CONTRA vcf results

    hi, i'm learning the CONTRA, by using the test datas downloaded on sourceforge, command as follows { Python-2.7/python CONTRA.v2.0.3/contra.py -t 0247401_D_BED_20090724_hg19_MERGED.bed -s P0667T_GATKrealigned_duplicates_marked.bam -c P0667N_GATKrealigned_duplicates_marked.bam -f human.fa -o outdir };
    here are the programs in it:
    1. Python-2.7
    2. R version 2.10.0
    3. samtools version: 0.1.8
    4. BEDTools-Version-2.11.2

    No error reports during processing(just like the web shows http://sourceforge.net/projects/cont.../Test%20files/). 3 files in the '/outdir/table' dir (CNATable.10rd.10bases.20bins.vcf; CNATable.10rd.10bases.20bins.DetailsFILTERED.txt; CNATable.10rd.10bases.20bins.txt), there are no cnv information in vcf file, only this description (
    ##fileformat=VCFv4.0
    ##reference=1000GenomesPilot-NCBI36
    ##INFO=<ID=SVTYPE,Number=1,Type=String,Description="Type of structural variant"
    ##INFO=<ID=END,Number=1,Type=Integer,Description="End position of the variant described in this record"
    ##ALT=<ID=CNV,Description="Copy number variable region"
    #CHROM POS ID REF ALT QUAL FILTER INFO)
    dose anyone know why?? thanks for help~
  • jmatés
    Member
    • Jan 2013
    • 14

    #2
    I'm at the same point... does anyone know what's going on?

    Comment

    • dawe
      Senior Member
      • Apr 2009
      • 258

      #3
      I had the same issue and wrote to the developer. He said he would have checked that but there have been no updates since then.
      I've tried to run CONTRA with python debugger, I remember there was a bug in some intermediate file that was not created, but then gave up and worked with bed-like outputs.

      d

      Comment

      • jmatés
        Member
        • Jan 2013
        • 14

        #4
        Originally posted by dawe View Post
        I had the same issue and wrote to the developer. He said he would have checked that but there have been no updates since then.
        I've tried to run CONTRA with python debugger, I remember there was a bug in some intermediate file that was not created, but then gave up and worked with bed-like outputs.

        d
        Thank you dawe.

        It's a pity. The program looks very nice and it fits perfectly to my bunch of samples! I've also written to the developer and well... waiting !

        Anyway if you could shed some light on how to work with CNVs with this "bed-like outputs" I'll be very grateful. One of the hardest things when you're a rookie (I think) is to have good judgment about which tools to use...

        Comment

        • mafonso
          Junior Member
          • Jul 2014
          • 4

          #5
          Hi,

          I have the same issue and can't figure out what the issue is. Did someone solve this problem? Is there another way to get the results without the .vcf file?

          Thanks

          Comment

          Latest Articles

          Collapse

          • GATTACAT
            Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
            by GATTACAT
            Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
            07-01-2026, 11:43 AM
          • SEQadmin2
            Nine Things a Sample Prep Scientist Thinks About Before Sequencing
            by SEQadmin2


            I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

            Here are nine questions we think about, in roughly the order they matter, before...
            06-18-2026, 07:11 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 07-02-2026, 11:08 AM
          0 responses
          12 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-30-2026, 05:37 AM
          0 responses
          15 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-26-2026, 11:10 AM
          0 responses
          20 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-17-2026, 06:09 AM
          0 responses
          54 views
          0 reactions
          Last Post SEQadmin2  
          Working...