ESTexplorer

[Triticum]

hi there!
i´ve read about the ESTexplorer, which is a web-based tool for pre/processing and annotating ESTs. so i tried it at http://estexplorer.els.mq.edu.au/estexplorer/ (i used the provided test-data) - but nothing happened! for 4 days now there is no progress in the status-report (still: "uploading data"). does anyone have experiences with this tool? does it really work?

Thanks. Triticum.

[mbritton]

My experience (about 18 months ago) was that when it worked, the progress was quite fast. However, I also had a similar experience to yours (no progress for days at one of the steps). I suggest you try resubmitting your sequences. Also, if you have another way to run CAP3 (or equivalent to build contigs), the annotation steps are more efficiently run directly with Blast2Go (http://www.blast2go.org/).

Monica Britton

[Triticum]

thanks a lot for your information. may be it would really be better if we use blast2go directly. i am just concerned about the computing-ressources, because we want to use 454-sequencing and so will have a huge amount of data. i thought that uploading to ESTexplore would provide a better performance for computing this amount of data, because currently we do not have a dedicated server for these calculations.

Triticum