Hi, I am a beginner working on SNP callings. I am trying samtools mpileup and it works pretty cool. However, I have no idea about the parameters and I am thinking which parameters control the output variance report. I can collect all the variances even that is sequencing error instead of SNPs. Thanks!
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Hi,
The samtools webpage has quite detailed explanation on the parameters. Here is the link: http://samtools.sourceforge.net/samtools.shtml.
Best regards,
Douglas
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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