I have been trying to check the correlation between the RNA-Seq RPKM and qPCR expr value.
Is it possible that the linear correlation between log2(RPKM) and log2(qPCR) value is worse than the linear correlation between RPKM and qPCR expression value consistently?
*qPCR expression value here is referring to normalized ct value by comparing with one gene (as shown in ncbi GSE5350)
Thank you.
Is it possible that the linear correlation between log2(RPKM) and log2(qPCR) value is worse than the linear correlation between RPKM and qPCR expression value consistently?
*qPCR expression value here is referring to normalized ct value by comparing with one gene (as shown in ncbi GSE5350)
Thank you.
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