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Quantifying antisense transcription from non-strand Specific RNA seq

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  • Quantifying antisense transcription from non-strand Specific RNA seq

    Hello All,

    I would like to learn more about how I can use non-strand specific RNA seq data to quantify antisense transcripts in mouse genome?

    I really appreciate any guidance and help.

    Thank you

  • #2
    If the libraries are not strand-specific, then it's impossible to distinguish sense from antisense.

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