Header Leaderboard Ad

Collapse

BWASW more reads in the output SAM file than in the input file

Collapse

Announcement

Collapse

SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
See more
See less
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • BWASW more reads in the output SAM file than in the input file

    When running BWA I get normal total number of reads (the same as in the input file).

    But when running BWASW alignment I get this:

    (that's from samtools flagstat)

    56125 + 0 in total (QC-passed reads + QC-failed reads)
    0 + 0 duplicates
    30118 + 0 mapped (53.66%:-nan%)
    0 + 0 paired in sequencing
    0 + 0 read1
    0 + 0 read2
    0 + 0 properly paired (-nan%:-nan%)
    0 + 0 with itself and mate mapped
    0 + 0 singletons (-nan%:-nan%)
    0 + 0 with mate mapped to a different chr
    0 + 0 with mate mapped to a different chr (mapQ>=5)

    My input number of reads loaded to BWA was 45997. How come I got over 80k?

  • #2
    BWA-SW performs local alignment and can output several alignments per input read/sequence. So a given read or different parts of a read can be aligned to different places, which are all included in the output file.

    Comment


    • #3
      I also heard that with extremely long reads bwa-sw can give not very reliable output. Is there any sense in browsing reads up to a given length, or it will change nothing, at will only consume my time.

      Comment

      Latest Articles

      Collapse

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 06-01-2023, 08:56 PM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-01-2023, 07:33 AM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-31-2023, 07:50 AM
      0 responses
      4 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-26-2023, 09:22 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Working...
      X