Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • gokhulkrishnakilaru
    Member
    • Jul 2011
    • 39

    edgeR error

    Hi friends, I am trying to analyse Differential expression using edgeR package and my WT and KO samples.

    When I issue the following code at the R prompt


    Code:
    out$p.adjust = p.adjust(out$p.value, method="BH")
    It gives the following error. Any ide?

    Code:
    Error in `$<-.data.frame`(`*tmp*`, "p.adjust", value = numeric(0)) : 
      replacement has 0 rows, data has 20434
    Calls: $<- -> $<-.data.frame
    Execution halted
    All helps appreciated.
  • Gordon Smyth
    Member
    • Apr 2011
    • 91

    #2
    Well, it would seem that your object 'out' doesn't contain a component called 'p.value'.

    Is it possible that you intended out$table$p.value? Since you don't tell us what 'out' is, I can only guess. Perhaps you might read the help function for the function that produced 'out'?

    This is not an error in the edgeR package. p.adjust() is not an edgeR function, and this looks like a user mistake anyway.

    Gordon

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    41 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    102 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    123 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    114 views
    0 reactions
    Last Post SEQadmin2  
    Working...