Hello,
The samples we sent to BGI were sequenced using illumina 1.5 pipeline so the expect read score should be ASCII 66-104. But among our reads, I found many bases are with quality score of ASCII 105. I think illumina only start to use a quality score of 41 from illumina 1.8 pipeline. So should I worry about that? Thx!
The samples we sent to BGI were sequenced using illumina 1.5 pipeline so the expect read score should be ASCII 66-104. But among our reads, I found many bases are with quality score of ASCII 105. I think illumina only start to use a quality score of 41 from illumina 1.8 pipeline. So should I worry about that? Thx!
Comment