Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • yjx1217
    Member
    • Oct 2011
    • 13

    #1

    quality score of 41 from illumina 1.5 pipeline?

    Hello,

    The samples we sent to BGI were sequenced using illumina 1.5 pipeline so the expect read score should be ASCII 66-104. But among our reads, I found many bases are with quality score of ASCII 105. I think illumina only start to use a quality score of 41 from illumina 1.8 pipeline. So should I worry about that? Thx!
  • Torst
    Senior Member
    • Apr 2008
    • 275

    #2
    No need to worry. It's probably correct. Most software will be happy with that.

    Comment

    • yjx1217
      Member
      • Oct 2011
      • 13

      #3
      Thank you very much!

      Originally posted by Torst View Post
      No need to worry. It's probably correct. Most software will be happy with that.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2




        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
        Today, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      22 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:51 AM
      0 responses
      14 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      67 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      47 views
      0 reactions
      Last Post SEQadmin2  
      Working...