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  • shuoguo
    Member
    • Sep 2012
    • 23

    ***samtools*** sort wrong???

    Hi i used samtools to sort a bam file like this:
    samtools sort my.bam my.out

    i get a new bam file, but i wanted the new bam file to be sorted at the third column (FNAME) but not right

    i check the result use

    samtools view my.out.bam | awk '{print $3}' | uniq

    what i got is something like:
    chr1
    chr10
    chr11
    ...
    chr2
    chr20
    ...
    chr3
    chr4

    I would like it to be:
    chr1
    chr2
    chr3
    ...

    thanks!!!
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    That's a valid way of sorting, just not what you want.

    I think Picard ReorderSam can reorder your .bam.

    Or, change your chromosome names so they are Chr01 instead of Chr1. Then the numbers will alphabetize the way you want them to.

    Comment

    • shuoguo
      Member
      • Sep 2012
      • 23

      #3
      thank you!

      Comment

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