Hi there,
I have DNA and RNA-seq data from pathogenic E. coli, on Illumina GA-IIx, 68 bp PE reads. There is no complete genome for the strains I used. Although several genomes from closely related strains have been closed, they vary in phage content. I am wondering if there is an assembler that will allow mapping of RNA-seq reads to a draft genome?
Sorry if this has already been covered, I have not found a thread.
Many thanks for your insight!
I have DNA and RNA-seq data from pathogenic E. coli, on Illumina GA-IIx, 68 bp PE reads. There is no complete genome for the strains I used. Although several genomes from closely related strains have been closed, they vary in phage content. I am wondering if there is an assembler that will allow mapping of RNA-seq reads to a draft genome?
Sorry if this has already been covered, I have not found a thread.
Many thanks for your insight!
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