Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • caswater
    Member
    • Jun 2011
    • 47

    #1

    question about cufflinks

    Just got result from cufflinks but not quite understand the output genes.fpkm_tracking:

    I used this command:

    Code:
    cufflinks -o Result -p 4 -g $GTF -b $RefGenome -u $file
    I then wish to check the estimated genes, but found only around 6000 genes (with known gene symbols) were reported in genes.fpkm_tracking, while the remaining are all with ids like CUFF.XXX, see below...

    So I was just wondering how I could know the expression of all ~20000 genes in the RTF annotation files, and CUFF.xxx corresponds to which gene?

    Thanks


    Code:
    tracking_id	class_code	nearest_ref_id	gene_id	gene_short_name	tss_id	locus	length	coverage	FPKM	FPKM_conf_lo	FPKM_conf_hi	FPKM_status
    OR4F5	-	-	OR4F5	-	-	chr1:69090-70008	-	-	0	0	0	OK
    CUFF.4	-	-	CUFF.4	-	-	chr1:109920-110286	-	-	1.57124	0.830549	2.31193	OK
    FAM138A	-	-	FAM138A	-	-	chr1:34610-36081	-	-	0	0	0	OK
    CUFF.6	-	-	CUFF.6	-	-	chr1:126628-127545	-	-	0.207493	0.0923964	0.322589	OK
    CUFF.5	-	-	CUFF.5	-	-	chr1:125397-125894	-	-	0.804427	0.435331	1.17352	OK
    CUFF.8	-	-	CUFF.8	-	-	chr1:167776-168206	-	-	1.66698	1.04788	2.28608	OK
    CUFF.10	-	-	CUFF.10	-	-	chr1:163967-164488	-	-	0.425551	0.168934	0.682169	OK
    CUFF.9	-	-	CUFF.9	-	-	chr1:169060-169232	-	-	1225.18	702.763	1747.6	OK
    CUFF.11	-	-	CUFF.11	-	-	chr1:227422-227796	-	-	7.54204	5.96942	9.11467	OK
    CUFF.12	-	-	CUFF.12	-	-	chr1:233147-233979	-	-	0.292227	0.146113	0.43834	OK
    CUFF.13	-	-	CUFF.13	-	-	chr1:234725-235592	-	-	0.379276	0.217552	0.540999	OK
    CUFF.14	-	-	CUFF.14	-	-	chr1:234090-234614	-	-	0.612381	0.306191	0.918572	OK

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...