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  • Tectona
    Member
    • Feb 2011
    • 11

    Retrieving sequences from CD-HIT clusters

    I have run CD-HIT on a set of DNA sequences (171 million). Now they are nicely clustered in a file ... but how can I efficiently retrieve all DNA sequences in clusters in particular frequency classes?
    There is a nice utility in CD-HIT (plot_len1.pl) which gives me a table with sequence frequencies for various length classes. So all the frequency information is in the .clstr file, but how do I get only the information out that I want... and how do I link that then back to the original sequences? Lets say I want to retrieve all sequences that occur from 10 to 19 times in my input dataset?

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