Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • geneart
    Member
    • Sep 2011
    • 42

    galaxy - illumina data analysis

    Hello folks,
    I am trying to use galaxy to run my analysis on my illumina data. I uploaded data into history and ran fastq groomer on it. I waited a day and still the job shows "waiting to be run" I have total 20GB data across 57 data sets:
    Just with the first data run , I am getting that message and still not changed the status! Any idea what is happening and when I could expect it to be done???I have written to galaxy people as well but any suggestions will help.

    Also is there a way to find out the datascore type of illumina if it is solexa or illumina 1.3-1.7 or 1.8+ ???
    Thanks
    geneart.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Galaxy has become a victim of their own success. Too many people like you around the world trying to use the main galaxy site. Of late advice from galaxy support has been to use an amazon cloud instance of galaxy (at your expense) if you have an immediate need for data analysis. Not much you can do but wait for the logjam to clear at main galaxy site.

    If that data is your own can't you check with the facility that generated the data as to what format the quality values are in. You could also run a tool like "fastqc" (http://www.bioinformatics.babraham.a...ojects/fastqc/) on one of the files and it should tell you about the quality format of the data.

    Comment

    • JackieBadger
      Senior Member
      • Mar 2009
      • 385

      #3
      Install a local instance of Galaxy on your computer and upload the data files as Admin. Then you can process much faster. The Galaxy install is straight forward with good instructions.
      Also, depending n what you want to do, its much faster using the original package outside of Galaxy in command line.

      Comment

      • geneart
        Member
        • Sep 2011
        • 42

        #4
        Thanks Genomax and JackieBadger ! I will definitely try the fastqc, and then run the rest of my data on galaxy local. Appreciate ur time

        Comment

        • geneart
          Member
          • Sep 2011
          • 42

          #5
          galaxy issue

          Hello all,
          can anyone suggest how to go about this please.....
          I created a workflow and ran the workflow on my dataset from the workflow menu,which opened up the interface "running workflow on dataset..." in the middle part of the screen.
          Fine and dandy ! I made changes as needed in the parameters and clicked on "run workflow". Now the problem was that the page showed error in that part of the screen indicating the page does not exist or retype address and such, the other two column displays showing the tools on my left of the screen and the current history did not change it was as it is .
          Now my question is: do u think my workflow would have initiated ? do I need to rerun my workflow?
          I checked to see after sometime and still there was nothing. But then my dataset is large so maybe it is not enough time yet to show any outputs, but I dont want to wait two days and then realise that my workflow di dnot run at all !!!!
          Please suggest
          Thanks,
          geneart.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
            by SEQadmin2


            Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

            The systematic characterization of the human proteome has
            ...
            07-20-2026, 11:48 AM
          • SEQadmin2
            Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
            by SEQadmin2



            Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
            ...
            07-09-2026, 11:10 AM
          • SEQadmin2
            Cancer Drug Resistance: The Lingering Barrier to Rising Survival
            by SEQadmin2



            Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

            There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
            07-08-2026, 05:17 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, Today, 12:17 PM
          0 responses
          10 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, Yesterday, 11:41 AM
          0 responses
          11 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 07-20-2026, 11:10 AM
          0 responses
          23 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 07-13-2026, 10:26 AM
          0 responses
          37 views
          0 reactions
          Last Post SEQadmin2  
          Working...