I'm running tophat for Arabidopsis RNA seq samples.
I've performed 2 runs; one with -x 20 (default) and the second time with -x 1, with the goal of using only uniquely mapped reads.
My question is; in which of the many reports inside the "logs" output folder should I look in order to compare between the 2 runs. So many reports I got confused, I can only see that the BAM files of the second run are smaller in size, as expected ????
Tanks
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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