[shaohua.fan]

Did you do random trimming or did you trim them down to the region with the highest information gain (which is what we do).

I think it had large genomes in mind. It is really good in RAM consumption and quite ok in thread usage and thus speed. Maybe CLC4 brings some scaffold capabilities?

Cheers,
björn

hi, björn
I totally agree with you that CLC is good at CPU and RAM control. But, i just wondering that why it doesn't support scaffolding which is important for genome assembly.

BTW, could you please tell me what is the region with highest information gain? Now i am just trim the reads randomly. But, i am thinking to use a sliding window to scan the region with less homopolymer(for example, the length of the homopolymer is < 4).

[usad]

Hi

just the most unambigous region. So if your whole 454 read aligns to one region and one region only to search for a short region in the read which also would allow placing it at this position only and not on another contig and use this as a pseudo-Illumina read.
But it probably doesn't help too much it will jut give you a few more links. (Maybe simulate first what you can expect, based on your N50 /aveage length or length distribution, linker length quality and number)

Cheers,
Björn

[Nomijill]

hi, björn
I totally agree with you that CLC is good at CPU and RAM control. But, i just wondering that why it doesn't support scaffolding which is important for genome assembly.

BTW, could you please tell me what is the region with highest information gain? Now i am just trim the reads randomly. But, i am thinking to use a sliding window to scan the region with less homopolymer(for example, the length of the homopolymer is < 4).

Just for everyone who is using the Genomics Workbench to know - There have been numerous updates to the functionality. Much of which is available as a plug-in download. For the de novo assembler there are two significant changes. 1 - The assembler now supports scaffolding of paired-end reads. 2 - You now have the ability to change the k-mer value as one of your parameters.

Happy Sequencing :-)
Naomi

[lmilne]

I am currently assembling about 215 gigabases of sequence data with clc_novo_assemble. Should I expect clc_novo_assemble to print its progress while it is running? Its last output of progress (80%) was two days ago.

[sklages]

I am currently assembling about 215 gigabases of sequence data with clc_novo_assemble. Should I expect clc_novo_assemble to print its progress while it is running? Its last output of progress (80%) was two days ago.

what does CLC support tell you? They are usually very helpful and responsive.

[Nomijill]

CLC bio Support

Please, whenever you have questions about the behavior of the CLC bio assemblers, contact [email protected]. You may also be interested in trying the new version of the assembler, if you have not already. The new algorithm scaffolds the paired end information.