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  • Mashin
    Junior Member
    • Aug 2012
    • 1

    #1

    PeakSeq problem

    I would appreciate any help or comment if anybody experienced same problem.

    I am trying to analyze ChIPseq data with PeakSeq v1.1 on MacOS.
    I provided mapability file from http://archive.gersteinlab.org/proj/...Map/H.sapiens/ and I created chromosome list file by putting chromosome names on separate line (chr1, chr2, chr3,..)
    Processing SAM file with -preprocess option seemed work fine and .bin files were created for each chromosome.
    The problem comes in next step when I try to run it with -peak_select option.
    It returns weird kind of error message:
    Code:
    PeakSeq -peak_select config.dat
     for reading.n Chromosome_list_HG.txt
    Anybody has an idea what can cause this?


    here is the config.dat file I am using:
    Code:
    # Experiment id is used as a prefix to the output file name.
    Experiment_id IP_vs_Inp
    
    # Chromosome ID list file, is used to generate data file paths.
    chromosome_list_file Chromosome_list_HG.txt
    
    # Enrichment fragment length For tag extension, this is the value of average fragment length.
    Enrichment_fragment_length 200
    
    # Target FDR in the simulations.
    target_FDR 0.05
    
    # Number of simulations performed while estimating the putative peaks.
    N_Simulations 10
    
    # Minimum distance between consecutive peaks
    Minimum_interpeak_distance 200
    
    # Mappability file that includes the uniquely mappable number of nucleotides per window for each chromosome.
    Mappability_map_file Mapability_HG.txt
    
    # The directory that contains the preprocessed ChIP-Seq reads, can specify multiple directories to pool reads from multiple source (e.g. replicates)
    ChIP_Seq_reads_data_dirs PeakSeq_IP
    
    # The directory that contains the preprocessed Input (control) experiment reads. (Multiple directories allowed)
    Input_reads_data_dirs PeakSeq_Inp
    
    # Seed for pseudo-random number generator. This is necessary for simulated background option (specified below).
    #Simulation_seed 1234567
    
    # Q-value threshold applied on the final set of peaks.
    max_Qvalue 0.05
    
    # There are currently two models for simulating the background for threshold selection
    # Simulated background is the simulation based method that is explained in the PeakSeq paper.
    # Poisson background uses a simple Poisson background with mean estimated from the read statistics. This option is still experimental but it is much faster than the simulated background option.
    # Background_model Poisson
    Background_model Simulated

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