I am running MACS14 within a University of Illinois Galaxy server and have some questions. How .wig files used over and above .bed files- if anyone has a good answer- I'd appreciate it.
1) Does anyone know how d affects the number of regions in the output?
2) If the 'fixed background lambda as local lambda for every peak region' box is not checked- I get a smaller number of regions specified. Should this be checked or not if I don't have a control?
3) If one specifies to build a shifting model- how does this affect your results?
Thanks very much for any help. I am an end user trying to look at data from a ChIP-Seq experiment.
1) Does anyone know how d affects the number of regions in the output?
2) If the 'fixed background lambda as local lambda for every peak region' box is not checked- I get a smaller number of regions specified. Should this be checked or not if I don't have a control?
3) If one specifies to build a shifting model- how does this affect your results?
Thanks very much for any help. I am an end user trying to look at data from a ChIP-Seq experiment.