What is the average coverage with good quality of whole genome sequence in read numbers (100 bp/read) and base pairs?
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Based on these experiments and their callability calculations, the authors estimate that generating 50x mapped coverage (60x before read mapping/filtering are applied) renders ~95% of the genome and ~81% of the exome callable. Intriguingly, however, the authors note that they’d sequenced an unrelated sample using the latest HiSeq chemistry and basecalling software, achieving the same level of callability with just 35x mapped coverage. If anything, this emphasizes that (as the authors suggest), a “callability” metric is far more informative to report when describing the resequencing of human genomes.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
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