Tweet
what does pooled sequensing mean? who can tell me? thanks very much!!
-
-
Pooled sequencing is a method to find variations that are typical of a population. You mix equimolar concentration of DNA samples from individuals of a population and without any barcoding you sequence it. -
thanks for your answer! can you give me a detailed description of the pooled sequensing?Comment
-
It's as TiborNagy said. When you are sequencing, instead of separating different samples in a lane by barcoding you just put them in in the same concentrations so as to get a list of differences seen within the pool of samples obtained from the same population. When you are doing the downstream analysis of the data, you do not know which mutation/polymorphism came from which sample but you know that it exists in the population and given enough samples you can predict the frequency of it.Originally posted by bewlib View Post
hope that helps!Comment
-
You can read more about this technique in:
and here:
Comment
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
Comment